Maricopa Community Colleges  HCE177   20042-20065 
Official Course Description: MCCCD Approval: 12/09/03
HCE177 20042-20065 LEC 3 Credit(s) 3 Period(s)
Histology Techniques Level 4
Identification of pathologic organisms and neurological structures. Use of stains for microorganisms and neurological structures. Explanation of theories of histochemical and immunohistochemical staining. Procedures for histochemical and immunohistochemical stains. Overview of specimens for electron microscopy and autopsy procedures. Emphasis on assessing histology workflow processes, laboratory procedure manuals, and workload and productivity recording and monitors. Prerequisites: HCE175 and HCE176. Corequisites: HCE178.
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MCCCD Official Course Competencies:
 
HCE177   20042-20065 Histology Techniques Level 4
1. Describe chemically stained pathologic organisms structure. (I)
2. Identify stains for microorganisms. (I)
3. Describe neurological tissue structures. (II)
4. Identify neurological stains. (II)
5. Describe theories of enzyme histochemical staining. (III)
6. Identify histochemical stains. (III)
7. Describe theories of immunohistochemical staining. (IV)
8. Identify immunohistochemical stains. (IV)
9. Review specimens for electron microscopy procedures. (V)
10. Review autopsy procedures. (VI)
11. Identify histology laboratory workflow processes. (VII)
12. Describe the use and function of laboratory procedure manuals. VII)
13. Describe workload recording, productivity recording, and monitors. VII)
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MCCCD Official Course Outline:
 
HCE177   20042-20065 Histology Techniques Level 4
    I. Microorganism Stains
        A. Bacteria
          1. Gram
          2. Giemsa
          3. Silver
        B. Mycobacteria
          1. Acid fast techniques
          2. Auramine-Rhodamine
        C. Spirochetes
          1. Dieterle
          2. Warthin-Starry
          3. Steiner
        D. Fungus and yeast
          1. Poly-Aromatic Sulfur Heterocycles (PASH)
          2. Methenamine silver techniques
          3. Mucicarmine
          4. Alcian blue
        E. Amoeba/protozoans
          1. Iron hematoxylin
          2. PASH
          3. Wheatley's
        F. Viruses (inclusion bodies)
          1. Lendrums
          2. Aldehyde fuchsin
      II. Neurological Tissue Structures
          A. Nerve fibers
            1. Bodian
            2. Biechowsky
            3. Sevier-Munger
            4. Holmes
          B. Myelin
            1. Luxol fast blue
            2. Weil
            3. Marchi
          C. Nissl
            1. Cresyl echt-violet
            2. Thionin
          D. Glial cells
            1. Helzer
            2. Cajal
            3. Phosphotungstic Acid Hematoxylin (PTAH)
        III. Enzyme Histochemical Staining
            A. Theories
            B. Techniques
              1. Metal salts
              2. Azo dyes
              3. Indoxyl
              4. Oxidation-reduction
            C. Applications
              1. Muscle
                a. Adenosine Triphosphatase (ATPase)
                b. Nicotinamide Adenine Dinucleotide (NADH)
                c. Succinate Dehydrogenase (SDH)
                d. Alpha-napthyl acetate esterase
                e. Alkaline phosphatase
                f. Acid phosphatase
                g. Phosphorylase
              2. Blood and bone marrow
                a. Chloroacetate esterase
                b. Acid phosphatase with/without tartrate
                c. Other esterases
          IV. Immunohistochemistry Staining
              A. Theory
              B. Staining methods
                1. Direct
                2. Indirect
                3. Peroxidase-antiperoxidase
                4. Avidin-biotin complex
              C. Dyes
                1. Fluorochromes
                2. Diaminobenzidine (DAB)
                3. Aminoethylcarbozole (AEC)
              D. Antibody dilution technique
            V. Electron Microscopy Procedures
                A. Fixation
                  1. Gluteraldehyde
                  2. Zamboni's
                  3. Osmium Tetroxide
                B. Processing
                  1. Dehydration
                  2. Plastics
                C. Microtomy
                  1. Thick sections
                  2. Thin sections
                D. Grid mounts/stains
              VI. Autopsy Procedures
                  A. Purpose
                  B. Technique
                    1. Gross examination
                    2. Microscopic examination
                    3. Histologic slide production
                VII. Operational Processes
                    A. Workflow
                    B. Procedure manuals C Staff scheduling
                    D. Daily oversight activities
                    E. Procedure and work standardization
                    F. Monitors
                      1. Workload
                      2. Productivity
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