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Official Course
Description: MCCCD Approval: 03/28/06 |
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CLS229 19906-20062
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L+L |
1 Credit(s) |
3 Period(s) |
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Clinical Parasitology |
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Laboratory handling and processing of clinical specimens for parasite examination. Emphasis on identification of parasites. Prerequisites: CLS118 or permission of instructor. |
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Go to Competencies Go to Outline
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MCCCD Official Course Competencies: |
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CLS229 19906-20062 |
Clinical Parasitology |
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1. |
List the types of clinical specimens that are submitted to the microbiology laboratory for parasite examination and explain how they are processed. (I) |
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2. |
Cite the advantages and disadvantages of various preservatives used in medical parasitology. (II) |
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3. |
Describe the procedure for and perform a macroscopic and microscopic examination of a stool specimen for parasites. (III) |
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4. |
Explain the preparation and fixation of fecal smears for permanent staining. (IV) |
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5. |
Describe the procedure for and calibrate a micrometer so that size determinations of parasites can be performed. (V) |
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6. |
List the information that should be included in a final report for parasite examination. (VI) |
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7. |
Identify common nonparasitic material as well as common parasites by observing prepared slides and wet mount material under the microscope.(VII) |
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Go to Description Go to top of Competencies
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MCCCD Official Course Outline: |
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CLS229 19906-20062 |
Clinical Parasitology |
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I. Collection of Clinical Specimens A. Feces B. Sigmoidoscopy material C. Duodenal contents D. Sputum E. Aspirates F. Biopsy material G. Scotch tape prep H. Blood films II. Preservation of Clinical Specimens A. Advantages and disadvantages of various preservatives B. Polyvinyl Alcohol (PVA) C. Schaudinn's D. 10% formalin E. Sodium acetate formalin (SAF) F. Methyolate iodine formaldehyde (MIF) III. Examination of Fecal Specimens A. Macroscopic examination 1. Physical examination 2. Age B. Microscopic examination 1. Direct wet mounts 2. Concentrations 3. Permanent stained slides 4. Culture IV. Permanently Stained Slides A. Preparation and fixation of slides B. Staining procedures and important steps 1. Trichrome 2. Iron hematoxylin V. Calibration of Micrometer for Determination of Size VI. Reporting Results of Parasite Examination VII. Identification of Nonparasitic and Parasitic Material A. Nonparasitic 1. Cellular exudates and pus cells 2. Charcot-Leyden crystals 3. Muscle fibers 4. Starch granules 5. Pollen grains 6. Yeast cells 7. Vegetable spirals 8. Vegetable hairs 9. Plant cells 10. Macrophages B. Parasitic 1. Amoebae 2. Flagellates 3. Ciliates 4. Plasmodium 5. Roundworms 6. Tapeworms 7. Flukes |