Official Course Description: MCCCD Approval: 11/22/94
RES120AB 19906-19946	LEC	1 Credit(s)	1 Period(s)
Applied Microbiology/ Decontamination
Classification of microorganisms. Decontamination of respiratory equipment. Protection of patient from hospital-caused infection. Prerequisites: None.

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MCCCD Official Course Competencies:
RES120AB   19906-19946	Applied Microbiology/ Decontamination

1.	Classify microorganisms. (I - XVIII)
2.	Describe procedures for cleaning and decontaminating respiratory care equipment. (XIX - XXI)
3.	Describe the procedures for protecting the patient from nosocomial infection. (XXII)

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MCCCD Official Course Outline:
RES120AB   19906-19946	Applied Microbiology/ Decontamination

I. Microorganisms by Cell Type A. Eukarotic (Protista) 1. algae (except blue-green) 2. protozoa 3. fungi 4. slime mold B. Prokaryotic (lower Protista) 1. blue-green algae 2. bacteria 3. rickettsiae 4. mycoplasmas II. Eurayotic Cell Structure A. Surface layers 1. cell membrane a. structure b. function 2. cell wall B. Nucleus 1. structure 2. function C. Cytoplasmic structures 1. endoplasmic reticulum a. structure b. function 2. mitochondria a. structure b. function 3. chloroplasts 4. Ribosomes a. structure b. function 5. Lysosomes a. structure b. function D. Motility organelles 1. cilia 2. flagella III. Prokaryotic Cell Structure A. Surface layers 1. cell membrane 2. cell wall a. gram-positive bacteria b. gram-negative bacteria B. Nucleus C. Cytoplasmic structures 1. multienzyme complexes 2. ribosomes 3. mesoseme 4. cytoplasmic granules D. Motility organelles 1. flagella 2. pili E. Capsule 1. description 2. functions 3. causative factors IV. Necessary Growth Conditions for Microorganisms A. Growth mediums B. Atmosphere 1. aerobic 2. anaerobic 3. facultative C. Temperature D. Osmotic pressure and pH E. Moisture F. Light V. Microbial Reproduction A. Asexual (binary fission) B. Sexual (conjugation) VI. Growth Pattern of Microorganisms A. Lag phase B. Exponential phase C. Stationary phase D. Death phase VII. Microbial Relationships A. Autotroph B. Heterotroph 1. saprophyte 2. parasite 3. symbiosis a. commensalism b. antibiosis c. ammensalism d. synnecrosis e. mutualism VIII. Spores IX. Microbial shapes A. Coccus 1. diplococcus 2. streptococcus 3. staphylococcus B. Bacillus C. Spirillum, spirochete D. Vibrios E. Fusiform X. Laboratory Examination Procedures Associated with Microorganisms A. Gram stain B. Acid fast stain C. Culture and sensitivity D. Cytology XI. Terms A. Inflammation B. Contamination C. Infection D. Pathogen E. Virulence F. Immunity G. Superinfection H. Pyrogenic I. Nosocomial infection J. Aerobic K. Anaerobic L. Toxins 1. exotoxin 2. endotoxin M. Vegetative cell XII. Collection of Respiratory Specimen for Laboratory Examination XIII. Common pathogenic microorganisms A. Bacillus B. Clostridium C. Corynebacerium D. Diplococcus E. Staphylococcus F. Streptococcus G. Mycobacterium H. Neisseria I. Proteus J. Pseudomonas K. Serratia L. Escherichia M. Klebsiella N. Hemophilus O. Salmonella XIV. Mycoplasma A. Structure B. Genus characteristics C. Species 1. location 2. diseases a. transmission b. treatment XV. Rickettsiae A. Structure B. Characteristics 1. shape 2. diseases a. clinical findings b. transmission c. treatment XVI. Viruses A. Structure 1. surface layers 2. nucleus 3. cytoplasmic structure 4. motility organelles B. Characteristics C. Species XVII. Fungi A. Structure B. Reproduction C. Types XVIII. Normal Flora A. Skin B. Respiratory tract C. Gastrointestinal tract XIX. Actions Involved in Equipment Decontamination A. Bacteriostatic B. Bacteriocidal C. Sterilization D. Disinfection E. Asepsis F. Antiseptic G. Decontamination H. Sanitization XX. Advantages, Disadvantages and Precautions Involved in Sterilization/Disinfection Systems A. Autoclaving B. Pasteurization C. Ethylene oxide D. Phenols E. Hexachlorophene F. Alcohols G. Iodine H. Chlorine and chlorine compounds I. Mercury compounds J. Quaternary ammonium compounds K. Formaldehyde L. Acid glutaraldehyde M. Alkaline glutaraldehyde XXI. Principles of Equipment Decontamination A. Humidifiers B. Nebulizers C. Tubing D. Ventilators E. Tents F. Regulators G. Flowmeters H. Spirometers I. Resuscitators J. Airways K. Electrical equipment L. Other related equipment XXII. Hospital Infection Control Procedures A. Handwashing in the control of nosocomial infections B. Isolation procedures C. Gowning and gloving technique D. Use of disposables in infection control E. Purpose and procedures of monitoring in infection control

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