Official Course Description: MCCCD Approval: 07/22/08
HCE163 19976-20086	L+L	1 Credit(s)	3 Period(s)
Beginning Hematology
Performance of complete blood counts with reading of differential slides limited to basic normal patterns. Performance of sedimentation rates. Overview of basic automated procedures. Prerequisites: None.

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MCCCD Official Course Competencies:
HCE163   19976-20086	Beginning Hematology

1.	Identify basic equipment utilized in the hematology section of the laboratory in a medical/clinical setting. (I)
2.	Utilize basic selected hematological testing equipment found in a medical/clinical setting. (I)
3.	Identify normal values for all procedures performed. (II)
4.	Perform unopette counts of white blood cells and thrombocytes. (III)
5.	Perform automated complete blood counts. (IV)
6.	Perform micro-hematocrit determination using micro-hematocrit centrifugation. (V)
7.	Perform erythrocyte sedimentation rates. (VI)
8.	Prepare acceptible slides for differential staining. (VII)
9.	Stain blood films using the Wright stain technique. (VIII)
10.	Perform basic normal differentials of Wright stained smears under supervision. (IX)
11.	Identify abnormal differentials to be referred to the appropriate medical/clinical practitioner for review. (IX)
12.	Describe and appropriately utilize controls when testing. (X)

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MCCCD Official Course Outline:
HCE163   19976-20086	Beginning Hematology

I. Equipment Identification A. Microscope and functions B. Hemacytometer C. Coulter counter D. Blood smear stains E. Unopettes F. Micro-Hematocrit Centrifuge and Reader G. Capillary tubes and sealing clay II. Normal Values A. Automated hemoglobin B. Automated red blood count (RBC) C. White Blood Count (WBC) D. Erythrocyte sedimentation rate E. Hematocrit F. Differential III. Unopette Counts of White Blood Count & Thrombocytes A. Principle B. Reagents C. Technique 1. Dilution 2. Charging the counting chamber 3. Counting D. Calculations 1. Formula 2. Estimations E. Sources of Error IV. Complete Automated Blood Cell Determinations A. Principle B. Reagents C. Technique D. Sources of Error V. Hematocrit Determination A. Principle B. Technique 1. Filling 2. Placement 3. Reading C. Correlations VI. Erythrocyte Sedimentation Rate Determination A. Principle B. Technique 1. Filling 2. Placement 3. Reading C. Sources of error VII. Slide Preparation A. Technique 1. Sample 2. Equipment B. Precautions 1. Time 2. Improper equipment 3. Sample viscosity C. Criteria 1. Thickness/thinness 2. Appropriate edge VIII. Staining Blood Films Using Wright Technique A. Principle 1. Reaction 2. Staining results B. Reagents 1. Preparation 2. Composition C. Technique 1. Fixing slide 2. Staining 3. Rinsing IX. Basic Normal Differential Cell Count A. Principle B. Procedure 1. Scan for even distribution of white blood cells 2. Count white blood cells 3. Examine red blood cells 4. Examine thrombocytes C. Recognize abnormal cells X. Controls A. Preferred usage B. Values

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