Maricopa Community Colleges  HCE162   19976-19985 
Official Course Description: MCCCD Approval: 03/25/97
HCE162 19976-19985 L+L 1 Credit(s) 3 Period(s)
Aseptic Techniques
Fundamentals of microbial control. Includes procedures for sanitation, disinfection, and sterilization. Specimen collection and handling. Compliance with Occupational Health and Safety Administration (OSHA) and the United States Center for Communicable Disease Control (CDC). Prerequisites: None.
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MCCCD Official Course Competencies:
 
HCE162   19976-19985 Aseptic Techniques
1. Define terms related to communicable diseases. (I)
2. Describe and apply selected types of sterilization and disinfection techniques. (II)
3. Identify the selected types of microorganisms and common methods of isolation. (III)
4. Apply principles of asepsis to hand washing, wound dressing., and disposal techniques (IV)
5. Apply principles of sterile techniques in preserving sterile packs, and creating and maintaining sterile field. (V)
6. Describe the principles of specimen collection, transport media, and storage. (VI)
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MCCCD Official Course Outline:
 
HCE162   19976-19985 Aseptic Techniques
    I. Communicable Diseases
        A. Definition
        B. Modes of transmission
        C. Concepts of body substance isolation
        D. Infection Control
          1. OSHA requirements
          2. CDC requirements
      II. Sterilization and Disinfection
          A. Definition
          B. Preparation of materials
          C. Procedures
            1. Autoclave
            2. Sterilizer
          D. Safety precautions
          E. Disposal
            1. Personal protection
            2. Guidelines and standards
        III. Microorganisms
            A. Classification of bacteria
              1. Gram positive
              2. Gram negative
            B. Morphology
            C. Representative pathogens
              1. Bacteria
              2. Virus
              3. Fungus
              4. Rickettsia
              5. Parasite
              6. Chlamydia
            D. Methods of isolation
          IV. Aseptic Techniques
              A. Hand washing
              B. Wound dressings
              C. Disposal techniques
            V. Sterile Techniques
                A. Preserve sterile packs
                B. Create and maintain a sterile field
              VI. Specimen Collection
                  A. Types
                    1. Throat culture
                    2. Urine
                    3. Feces
                    4. Vaginal
                    5. Rectal
                  B. Collection and handling
                    1. Throat swab
                    2. Throat culture
                    3. Urine culture for colony count
                    4. Hemoccult test
                    5. Potassium hydroxide (KOH) prep
                    6. Gram stain
                  C. Transport media
                    1. Types
                    2. Other transport systems
                  D. Storage
                    1. Shelf life
                    2. Temperature
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