Maricopa Community Colleges  HCE161   19976-20086 
Official Course Description: MCCCD Approval: 07/22/08
HCE161 19976-20086 L+L 1 Credit(s) 3 Period(s)
Basic Skills in Clinical Microbiology
An introduction to basic microbiology. Topics include causative agents of infection, spread of infectious agents, morphology of bacteria, sterilization techniques, collection of clinical specimens, gram staining, organisms common to human flora and sensitivity testing.
Prerequisites: None.
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MCCCD Official Course Competencies:
 
HCE161   19976-20086 Basic Skills in Clinical Microbiology
1. List and define the types of microorganisms that can cause infections. (I)
2. Describe the manners in which organisms can invade the body. (II)
3. Describe the morphological types of bacteria. (III)
4. Describe infection control by utilizing procedures such as disinfection, sterilization and aseptic techniques. (IV)
5. Describe the general principles that should be followed when collecting clinical specimens for examination and/or culture. (V)
6. Explain how the integrity of clinical specimens can be maintained until they are processed. (V)
7. Achieve competent streaking/culturing techniques. (IV)
8. Perform the gram stain and interpret the results. (VI)
9. Perform and interpret the differential tests used to identify selected gram positive cocci. (VII)
10. Describe the significance of selected gram negative bacilli. (VII)
11. Perform the agar disk diffusion susceptibility test. (VIII)
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MCCCD Official Course Outline:
 
HCE161   19976-20086 Basic Skills in Clinical Microbiology
    I. Organisms capable of causing infections and disease
        A. Bacteria
        B. Mycoplasma
        C. Rickettsia
        D. Chlamydia
        E. Viruses
        F. Parasites
        G. Fungi
      II. Bacterial Invasion, Infection, and Disease
          A. Invasion of organisms
          B. Types of infections
          C. Types of diseases
        III. Microscopic Morphology of Bacteria
            A. Spherical
            B. Cylindrical
            C. Spiral
          IV. Disinfection, Sterilization, and Aseptic Techniques
              A. Disinfection
                1. Definitions
                2. Factors affecting disinfection
                3. Types of disinfectants
              B. Sterilization
                1. Preparation of materials
                2. Methodology
                  a. Gas
                  b. Heat
                  c. Filtration
                  d. Radiation
                3. Equipment and procedures
              C. Aseptic techniques
                1. Reasons for aseptic technique
                2. Basic rules to follow to achieve asepsis
                3. Transferring and streaking of bacteria
                4. Disposal of contaminated materials
            V. Specimen Collection and Handling
                A. General principles for specimen collection
                B. Equipment needed for the proper collection and transport of clinical specimens
                  1. Swabs
                  2. Containers
                  3. Transport media
                C. Collection techniques for various specimens which include:
                  1. Blood
                  2. Respiratory tract
                  3. Urine
                  4. Cerebral spinal fluid
                  5. Ear and eye
                  6. Wounds
                  7. Genital
                  8. Feces
                  9. Tissue and biopsy material
                  10. Fungus studies
                  11. Viral studies
              VI. Staining
                  A. Purpose of staining
                  B. Preparation of bacterial smears
                  C. Gram stain procedure
                VII. Common Microbes of Man and their Identification
                    A. Common cocci of man
                      1. Common tests for their identification
                      2. Flow chart of aerobic cocci
                    B. Common gram negative bacilli
                  VIII. Agar Disk Diffusion Susceptibility Test Procedure
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