MCCCD Official Course Competencies:

HCE178  2006 Fall – 2011 Summer II

Practicum: Cellular Biological and Immunohistochemical Staining

1.

Identify chemically stained pathologic organisms structures. (I)

2.

Use stains for microorganisms. (I)

3.

Identify neurological tissue structures. (II)

4.

Use neurological stains. (II)

5.

Apply theories of enzyme histochemical staining. (III)

6.

Use histochemical stains. (III)

7.

Apply theories of immunohistochemical staining. (IV)

8.

Use immunohistochemical stains. (IV)

9.

Observe and report on electron microscopy procedures. (V)

10.

Observe and report on autopsy procedures. (VI)

11.

Assess histology laboratory workflow processes. (VII)

12.

Use laboratory procedure manuals. (VII)

13.

Apply workload recording, productivity recording, and monitors. (VII)

MCCCD Official Course Outline:

HCE178  2006 Fall – 2011 Summer II

Practicum: Cellular Biological and Immunohistochemical Staining

I. Microorganism Stains

A. Bacteria

1. Gram

2. Giemsa

3. Silver

B. Mycobacteria

1. Acid fast techniques

2. Auramine-Rhodamine

C. Spirochetes

1. Dieterle

2. Warthin-Starry

3. Steiner

D. Fungus and yeast

1. Poly-Aromatic Sulfur Heterocycles (PASH)

2. Methenamine silver techniques

3. Mucicarmine

4. Alcian blue

E. Amoeba/protozoans

1. Iron hematoxylin

2. PASH

3. Wheatley's

F. Viruses (inclusion bodies)

1. Lendrums

2. Aldehyde fuchsin

II. Neurological Tissue Structures

A. Nerve fibers

1. Bodian

2. Biechowsky

3. Sevier-Munger

4. Holmes

B. Myelin

1. Luxol fast blue

2. Weil

3. Marchi

C. Nissl

1. Cresyl echt-violet

2. Thionin

D. Glial cells

1. Helzer

2. Cajal

3. Phosphoungstic Acid Haematoxylin (PTAH)

III. Enzyme Histochemical Staining

A. Theories

B. Techniques

1. Metal salts

2. Azo dyes

3. Indoxyl

4. Oxidation-reduction

C. Applications

1. Muscle

a. Adenosine Triphosphatase (ATPase)

b. Nicotinamide Adenine Dinucleotide (NADH)

c. Succinate Dehydrogenase (SDH)

d. Alpha-napthyl acetate esterase

e. Alkaline phosphatase

f. Acid phosphatase

g. Phosphorylase

2. Blood and bone marrow

a. Chloroacetate esterase

b. Acid phosphatase with/without tartrate

c. Other esterases

IV. Immunohistochemistry Staining

A. Theory

B. Staining methods

1. Direct

2. Indirect

3. Peroxidase-antiperoxidase

4. Avidin-biotin complex

C. Dyes

1. Fluorochromes

2. Diaminobenzidine (DAB)

3. Aminoethylcarbozole (AEC)

D. Antibody dilution technique

V. Electron Microscopy Procedures

A. Fixation

1. Gluteraldehyde

2. Zamboni's

3. Osmium Tetroxide

B. Processing

1. Dehydration

2. Plastics

C. Microtomy

1. Thick sections

2. Thin sections

D. Grid mounts/stains

VI. Autopsy Procedures

A. Purpose

B. Technique

1. Gross examination

2. Microscopic examination

3. Histologic slide production

VII. Operational Processes

A. Workflow

B. Procedure manuals

C. Staff scheduling

D. Daily oversight activities

E. Procedure and work standardization

F. Monitors

1. Workload

2. Productivity