MCCCD Official Course Competencies:

HCE177  2006 Fall – 2011 Summer II

Cellular Biological and Immunohistochemical Staining

1.

Describe chemically stained pathologic organisms structure. (I)

2.

Identify stains for microorganisms. (I)

3.

Describe neurological tissue structures. (II)

4.

Identify neurological stains. (II)

5.

Describe theories of enzyme histochemical staining. (III)

6.

Identify histochemical stains. (III)

7.

Describe theories of immunohistochemical staining. (IV)

8.

Identify immunohistochemical stains. (IV)

9.

Review specimens for electron microscopy procedures. (V)

10.

Review autopsy procedures. (VI)

11.

Identify histology laboratory workflow processes. (VII)

12.

Describe the use and function of laboratory procedure manuals. (VII)

13.

Describe workload recording, productivity recording, and monitors. (VII)

MCCCD Official Course Outline:

HCE177  2006 Fall – 2011 Summer II

Cellular Biological and Immunohistochemical Staining

I. Microorganism Stains

A. Bacteria

1. Gram

2. Giemsa

3. Silver

B. Mycobacteria

1. Acid fast techniques

2. Auramine-Rhodamine

C. Spirochetes

1. Dieterle

2. Warthin-Starry

3. Steiner

D. Fungus and yeast

1. Poly-Aromatic Sulfur Heterocycles (PASH)

2. Methenamine silver techniques

3. Mucicarmine

4. Alcian blue

E. Amoeba/protozoans

1. Iron hematoxylin

2. PASH

3. Wheatley's

F. Viruses (inclusion bodies)

1. Lendrums

2. Aldehyde fuchsin

II. Neurological Tissue Structures

A. Nerve fibers

1. Bodian

2. Biechowsky

3. Sevier-Munger

4. Holmes

B. Myelin

1. Luxol fast blue

2. Weil

3. Marchi

C. Nissl

1. Cresyl echt-violet

2. Thionin

D. Glial cells

1. Helzer

2. Cajal

3. Phosphotungstic Acid Hematoxylin (PTAH)

III. Enzyme Histochemical Staining

A. Theories

B. Techniques

1. Metal salts

2. Azo dyes

3. Indoxyl

4. Oxidation-reduction

C. Applications

1. Muscle

a. Adenosine Triphosphatase (ATPase)

b. Nicotinamide Adenine Dinucleotide (NADH)

c. Succinate Dehydrogenase (SDH)

d. Alpha-napthyl acetate esterase

e. Alkaline phosphatase

f. Acid phosphatase

g. Phosphorylase

2. Blood and bone marrow

a. Chloroacetate esterase

b. Acid phosphatase with/without tartrate

c. Other esterases

IV. Immunohistochemistry Staining

A. Theory

B. Staining methods

1. Direct

2. Indirect

3. Peroxidase-antiperoxidase

4. Avidin-biotin complex

C. Dyes

1. Fluorochromes

2. Diaminobenzidine (DAB)

3. Aminoethylcarbozole (AEC)

D. Antibody dilution technique

V. Electron Microscopy Procedures

A. Fixation

1. Gluteraldehyde

2. Zamboni's

3. Osmium Tetroxide

B. Processing

1. Dehydration

2. Plastics

C. Microtomy

1. Thick sections

2. Thin sections

D. Grid mounts/stains

VI. Autopsy Procedures

A. Purpose

B. Technique

1. Gross examination

2. Microscopic examination

3. Histologic slide production

VII. Operational Processes

A. Workflow

B. Procedure manuals

C. Staff scheduling

D. Daily oversight activities

E. Procedure and work standardization

F. Monitors

1. Workload

2. Productivity