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Official Course
Description: MCCCD Approval: 4-25-2006 |
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HCE176 2006 Fall
– 2011 Summer II |
LAB 3.0 Credit(s) 6.0 Period(s) 0.0 Load Occ |
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Practicum: Cellular Biological Staining |
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Practicum in histology techniques performed in an anatomic laboratory. Recognition of basic cellular structure, carbohydrate structures, connective tissue, lipid structures, and pigments and minerals in tissues. Application of chemical staining. Emphasis on stains for nuclear and cytoplasmic structures, carbohydrates and lipids, connective tissue, and tissue pigments and minerals stains. Prerequisites: HCE173 and HCE174. Corequisites: HCE175. |
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Go to Competencies Go to Outline
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MCCCD Official Course Competencies: |
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HCE176 2006 Fall – 2011 Summer II |
Practicum: Cellular
Biological Staining |
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1. |
Identify basic cellular structure. (I) |
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2. |
Apply theory of chemical staining. (II) |
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3. |
Use nuclear and cytoplasmic stains. (III) |
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4. |
Identify carbohydrates in tissue structures. (IV) |
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5. |
Use carbohydrate stains. (IV) |
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6. |
Identify connective tissue structures. (V) |
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Use connective tissue stains. (V) |
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8. |
Identify lipid structures in tissue. (VI) |
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9. |
Use lipid stains. (VI) |
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10. |
Identify pigments and minerals found in tissue. (VII) |
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11. |
Use pigment and mineral stains. (VII) |
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12. |
Describe minerals found in tissue. (VIII) |
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13. |
Identify mineral stains. (VIII) |
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14. |
Describe cytoplasmic granules found in tissue. (IX) |
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15. |
Identify cytoplasmic granules found in stains. (IX) |
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Go to Description Go to top of Competencies
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MCCCD Official Course Outline: |
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HCE176 2006 Fall – 2011 Summer II |
Practicum: Cellular
Biological Staining |
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I. Cellular structure A. Nucleus B. Cytoplasm II. Theory of Chemical Staining A. Nuclear B. Cytoplasmic C. Natural dyes 1. Indigo 2. Cochineal 3. Orcein 4. Hematoxylin D. Artificial/synthetic dyes E. Chemical mechanisms 1. Differentiation 2. Acid and basic dye classification 3. Oxidation and reduction 4. Auxochromes and chromatophores 5. Absorption/solution theory 6. Metachromatic staining 7. Mordants 8. Electrostatic bonds III. Nuclear and Cytoplasmic Stains A. Nuclear 1. Hematoxylin a. Alum b. Iron c. Other 2. Methylene blue 3. Celestine blue 4. Carmine B. Cytoplasmic 1. Eosin 2. Phloxine C. Combination 1. Hematoxylin and eosin 2. Giemsa 3. Methyl green-pyronin IV. Carbohydrates A. Carbohydrates classifications in tissue structures 1. Polysaccharides 2. Neutral mucins 3. Acid mucins 4. Sulfated and non-sulfated mucins 5. Mucoproteins 6. Glycoproteins B. Carbohydrate stains 1. Periodic acid Schiff (PAS) 2. Mucicarmine 3. Alcian blue stains 4. Colloidal iron stains 5. Congo red 6. Thioflavin T 7. Crystal violet/methyl violet V. Connective Tissue A. Collagen 1. Trichrome staining 2. Other B. Reticulum 1. Wilder 2. Gridley 3. Snook 4. Laidlaw C. Elastic 1. Verhoeff van Gieson 2. Aldehyde fuchsin 3. Orcein 4. Resorcin fuchsin D. Basement membranes 1. Jones 2. Gomori's PAS – Methenamine silver E. Muscle 1. Phosphotungstic Acid Hematoxylin (PTAH) 2. Trichromes 3. Lendrum VI. Lipids A. Tissue structures 1. Storage cells 2. Trauma displacement 3. Cellular degeneration B. Stains 1.
2. Oil red O VII. Pigments A. Artifact 1. Formalin 2. Mercury 3. Chrome B. Exogenous 1. Carbon 2. Asbestos 3. Tattoo C. Endogenous hematogenous 1. Hemosiderin a. Prussian blue stain b. Turnbull's stain 2. Bile a. Hall's b. Other D. Endogenous nonhematogenous 1. Melanin a. Fontana-Masson b. Schmorl 2. Argyrophil granules a. Grimelius argyrophil stain b. Sevier-Munger c. Churukian-Schenk 3. Lipidic pigments a. Lipofucscin b. Ceroid VIII. Minerals A. Endogenous deposits 1. Urates – Gomori's methenamine silver 2. Other B. Calcium 1. Von Kossa 2. Alizarin red S C. Copper 1. Rhodanine 2. Rubeanic acid IX. Cytoplasmic Granules A. Chromaffin granules 1. Schmorl 2. Other B. Argentaffin granules 1.
2. Other |