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Official Course
Description: MCCCD Approval:
4-25-2006 |
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HCE171 2006
Fall – 2011 Summer II |
LEC 2.0 Credit(s) 2.0 Period(s) 2.0 Load Occ |
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Chemistry of Fixation |
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Principles of fixation and fixative solutions. Anatomic laboratory instrumentation and specimen handling, identification, and tracking protocols. Emphasis on gross examination processes, tissue processing protocols, and specialized processing techniques. Prerequisites: Admission to Histologic Technology Program or permission of Program Director. Corequisites: HCE170 and HCE172. |
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Go to Competencies Go to Outline
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MCCCD Official Course Competencies: |
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HCE171 2006 Fall – 2011 Summer II |
Chemistry of Fixation |
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1. |
Identify anatomic laboratory instrumentation. (I) |
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Describe basic specimen acquisition and handling protocols. (II) |
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Describe specimen identification and tracking protocols. (II) |
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Review gross examination processes. (III) |
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Describe the basic principles of fixation. (IV) |
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Identify the components, properties, and actions of fixative solutions. (IV) |
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Describe the stages and chemicals used in routine paraffin tissue processing protocols. (V) |
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Review specialized processing techniques. (VI) |
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Go to Description Go to top of Competencies
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MCCCD Official Course Outline: |
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HCE171 2006 Fall – 2011 Summer II |
Chemistry of Fixation |
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I. Instrumentation A. Dissection equipment B. Tissue processors C. Embedding stations/centers D. Microtomes E. Automated labeling systems F. Automated staining systems G. Automated coverslippers II. Specimen Acquisition and Handling A. Tracking protocols B. Identification protocols III. Gross Examination Processes A. Dissection criteria B. Sample selection C. Orientation basics IV. Fixative Solutions and Fixation Principles A. Aldehyde fixatives 1. Components 2. Properties 3. Histologic actions B. Mercuric and heavy metal fixatives 1. Components 2. Properties 3. Histologic actions C. Chromate fixatives 1. Components 2. Properties 3. Histologic actions D. Picric fixatives 1. Components 2. Properties 3. Histologic actions E. Non-aqueous fixatives 1. Components 2. Properties 3. Histologic actions F. Proprietary fixatives 1. Components 2. Properties 3. Histologic actions V. Paraffin Tissue Processing Protocols A. Processing stages 1. Fixation 2. Dehydration 3. Clearing 4. Infiltration B. Factors affecting processing C. Chemicals 1. Dehydration agents a. Alcohols b. Acetone c. Dioxane d. Cellusolve e. Tetrahydrofuran f. Proprietary formulations 2. Clearing agents a. Xylene/toluene b. Benzene c. Xylene substitutes 3. Paraffin infiltration D. Tissue processors 1. Processing schedules 2. Maintenance 3. Errors in processing E. Alternate infiltration agents 1. Water soluble waxes 2. Celloidin 3. Double embedding methods and frozen sections 4. Resins and plastics VI. Specialized Processing Techniques A. Electron microscopy B. Other |