Maricopa Community Colleges  HCE178   20042-20065 
Official Course Description: MCCCD Approval: 12/09/03
HCE178 20042-20065 LAB 3 Credit(s) 6 Period(s)
Histology Techniques Level 4 Practicum
Practicum in histology techniques performed in an anatomic laboratory. Identification of pathologic organisms and neurological structures. Use of stains for microorganisms and neurological structures. Application of histochemical and immunohistochemical stains. Observation of electron microscopy and autopsy procedures. Emphasis on histology workflow processes, laboratory procedure manuals, and workload and productivity recording and monitors. Prerequisites: HCE175 and HCE176. Corequisites: HCE177.
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MCCCD Official Course Competencies:
 
HCE178   20042-20065 Histology Techniques Level 4 Practicum
1. Identify chemically stained pathologic organisms structures. (I)
2. Use stains for microorganisms. (I)
3. Identify neurological tissue structures. (II)
4. Use neurological stains. (II)
5. Apply theories of enzyme histochemical staining. (III)
6. Use histochemical stains. (III)
7. Apply theories of immunohistochemical staining. (IV)
8. Use immunohistochemical stains. (IV)
9. Observe and report on electron microscopy procedures. (V)
10. Observe and report on autopsy procedures. (VI)
11. Assess histology laboratory workflow processes. (VII)
12. Use laboratory procedure manuals. (VII)
13. Apply workload recording, productivity recording, and monitors. (VII)
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MCCCD Official Course Outline:
 
HCE178   20042-20065 Histology Techniques Level 4 Practicum
    I. Microorganism Stains
        A. Bacteria
          1. Gram
          2. Giemsa
          3. Silver
        B. Mycobacteria
          1. Acid fast techniques
          2. Auramine-Rhodamine
        C. Spirochetes
          1. Dieterle
          2. Warthin-Starry
          3. Steiner
        D. Fungus and yeast
          1. Poly-Aromatic Sulfur Heterocycles (PASH)
          2. Methenamine silver techniques
          3. Mucicarmine
          4. Alcian blue
        E. Amoeba/protozoans
          1. Iron hematoxylin
          2. PASH
          3. Wheatley's
        F. Viruses (inclusion bodies)
          1. Lendrums
          2. Aldehyde fuchsin
      II. Neurological Tissue Structures
          A. Nerve fibers
            1. Bodian
            2. Biechowsky
            3. Sevier-Munger
            4. Holmes
          B. Myelin
            1. Luxol fast blue
            2. Weil
            3. Marchi
          C. Nissl
            1. Cresyl echt-violet
            2. Thionin
          D. Glial cells
            1. Helzer
            2. Cajal
            3. Phosphoungstic Acid Haematoxylin (PTAH)
        III. Enzyme Histochemical Staining
            A. Theories
            B. Techniques
              1. Metal salts
              2. Azo dyes
              3. Indoxyl
              4. Oxidation-reduction
            C. Applications
              1. Muscle
                a. Adenosine Triphosphatase (ATPase)
                b. Nicotinamide Adenine Dinucleotide (NADH)
                c. Succinate Dehydrogenase (SDH)
                d. Alpha-napthyl acetate esterase
                e. Alkaline phosphatase
                f. Acid phosphatase
                g. Phosphorylase
              2. Blood and bone marrow
                a. Chloroacetate esterase
                b. Acid phosphatase with/without tartrate
                c. Other esterases
          IV. Immunohistochemistry Staining
              A. Theory
              B. Staining methods
                1. Direct
                2. Indirect
                3. Peroxidase-antiperoxidase
                4. Avidin-biotin complex
              C. Dyes
                1. Fluorochromes
                2. Diaminobenzidine (DAB)
                3. Aminoethylcarbozole (AEC)
              D. Antibody dilution technique
            V. Electron Microscopy Procedures
                A. Fixation
                  1. Gluteraldehyde
                  2. Zamboni's
                  3. Osmium Tetroxide
                B. Processing
                  1. Dehydration
                  2. Plastics
                C. Microtomy
                  1. Thick sections
                  2. Thin sections
                D. Grid mounts/stains
              VI. Autopsy Procedures
                  A. Purpose
                  B. Technique
                    1. Gross examination
                    2. Microscopic examination
                    3. Histologic slide production
                VII. Operational Processes
                    A. Workflow
                    B. Procedure manuals
                    C. Staff scheduling
                    D. Daily oversight activities
                    E. Procedure and work standardization
                    F. Monitors
                      1. Workload
                      2. Productivity
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