Official Course Description: MCCCD Approval: 12/09/03
HCE176 20042-20065	LAB	3 Credit(s)	6 Period(s)
Histology Techniques Level 3 Practicum
Practicum in histology techniques performed in an anatomic laboratory. Recognition of basic cellular structure, carbohydrate structures, connective tissue, lipid structures, and pigments and minerals in tissues. Application of chemical staining. Emphasis on stains for nuclear and cytoplasmic structures, carbohydrates and lipids, connective tissue, and tissue pigments and minerals stains. Prerequisites: HCE173 and HCE174. Corequisites: HCE175.

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MCCCD Official Course Competencies:
HCE176   20042-20065	Histology Techniques Level 3 Practicum

1.	Identify basic cellular structure.(I)
2.	Apply theory of chemical staining. (II)
3.	Use nuclear and cytoplasmic stains.(III)
4.	Identify carbohydrates in tissue structures.(IV)
5.	Use carbohydrate stains.(IV)
6.	Identify connective tissue structures.(V)
7.	Use connective tissue stains. (V)
8.	Identify lipid structures in tissue.(VI)
9.	Use lipid stains.(VI)
10.	Identify pigments and minerals found in tissue.(VII)
11.	Use pigment and mineral stains.(VII)
12.	Describe minerals found in tissue (VIII)
13.	Identify mineral stains. (VIII)
14.	Describe cytoplasmic granules found in tissue. (IX)
15.	Identify cytoplasmic granules found in stains. (IX)

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MCCCD Official Course Outline:
HCE176   20042-20065	Histology Techniques Level 3 Practicum

I. Cellular structure A. Nucleus B. Cytoplasm II. Theory of Chemical Staining A. Nuclear B. Cytoplasmic C. Natural dyes 1. Indigo 2. Cochineal 3. Orcein 4. Hematoxylin D. Artificial/synthetic dyes E. Chemical mechanisms 1. Differentiation 2. Acid and basic dye classification 3. Oxidation and reduction 4. Auxochromes and chromatophores 5. Absorption/solution theory 6. Metachromatic staining 7. Mordants 8. Electrostatic bonds III. Nuclear and Cytoplasmic Stains A. Nuclear 1. Hematoxylin a. Alum b. Iron c. Other 2. Methylene blue 3. Celestine blue 4. Carmine B. Cytoplasmic 1. Eosin 2. Phloxine C. Combination 1. Hematoxylin and eosin 2. Giemsa 3. Methyl green-pyronin IV. Carbohydrates A. Carbohydrates classifications in tissue structures 1. Polysaccharides 2. Neutral mucins 3. Acid mucins 4. Sulfated and non-sulfated mucins 5. Mucoproteins 6. Glycoproteins B. Carbohydrate stains 1. Periodic acid Schiff (PAS) 2. Mucicarmine 3. Alcian blue stains 4. Colloidal iron stains 5. Congo red 6. Thioflavin T 7. Crystal violet/methyl violet V. Connective Tissue A. Collagen 1. Trichrome staining 2. Other B. Reticulum 1. Wilder 2. Gridley 3. Snook 4. Laidlaw C. Elastic 1. Verhoeff van Gieson 2. Aldehyde fuchsin 3. Orcein 4. Resorcin fuchsin D. Basement membranes 1. Jones 2. Gomori's PAS – Methenamine silver E. Muscle 1. Phosphotungstic Acid Hematoxylin (PTAH) 2. Trichromes 3. Lendrum VI. Lipids A. Tissue structures 1. Storage cells 2. Trauma displacement 3. Cellular degeneration B. Stains 1. Sudan black 2. Oil red O VII. Pigments A. Artifact 1. Formalin 2. Mercury 3. Chrome B. Exogenous 1. Carbon 2. Asbestos 3. Tattoo C. Endogenous hematogenous 1. Hemosiderin a. Prussian blue stain b. Turnbull's stain 2. Bile a. Hall's b. Other D. Endogenous nonhematogenous 1. Melanin a. Fontana-Masson b. Schmorl 2. Argyrophil granules a. Grimelius argyrophil stain b. Sevier-Munger c. Churukian-Schenk 3. Lipidic pigments a. Lipofucscin b. Ceroid VIII. Minerals A. Endogenous deposits 1. Urates – Gomori's methenamine silver 2. Other B. Calcium 1. Von Kossa 2. Alizarin red S C. Copper 1. Rhodanine 2. Rubeanic acid IX. Cytoplasmic Granules A. Chromaffin granules 1. Schmorl 2. Other B. Argentaffin granules 1. Fontana Masson 2. Other

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