Official Course Description: MCCCD Approval: 12/09/03
HCE175 20042-20065	LEC	3 Credit(s)	3 Period(s)
Histology Techniques Level 3
Recognition of basic cellular structure. Identification of carbohydrates classification, pigments, minerals, and lipids in tissues and connective tissue structures. Explanation of chemical staining theory. Stain principles and procedures for nuclear and cytoplasmic structures, carbohydrates and lipids, connective tissue, and tissue pigments and minerals stains. Prerequisites: HCE173 and HCE174. Corequisites: HCE176.

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MCCCD Official Course Competencies:
HCE175   20042-20065	Histology Techniques Level 3

1.	Review basic cellular structure.(I)
2.	Describe theory of chemical staining. (II)
3.	Identify nuclear and cytoplasmic stains.(III)
4.	Describe the classification of carbohydrates in tissue structures.(IV)
5.	Identify carbohydrate stains.(IV)
6.	Describe connective tissue structures.(V)
7.	Identify connective tissue stains. (V)
8.	Describe lipid structures in tissue. (VI)
9.	Identify lipid stains. (VI)
10.	Describe pigments found in tissue. (VII)
11.	Identify pigments stains. (VII)
12.	Describe minerals found in tissue (VIII)
13.	Identify mineral stains. (VIII)
14.	Describe cytoplasmic granules found in tissue. (IX)
15.	Identify cytoplasmic granules found in stains. (IX)

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MCCCD Official Course Outline:
HCE175   20042-20065	Histology Techniques Level 3

I. Cellular Structure A. Nucleus B. Cytoplasm II. Theory of Chemical Staining A. Nuclear B. Cytoplasmic C. Natural dyes 1. Indigo 2. Cochineal 3. Orcein 4. Hematoxylin D. Artificial/synthetic dyes E. Chemical mechanisms 1. Differentiation 2. Acid and basic dye classification 3. Oxidation and reduction 4. Auxochromes and chromatophores 5. Absorption/solution theory 6. Metachromatic staining 7. Mordants 8. Electrostatic bonds III. Nuclear and Cytoplasmic Stains A. Nuclear 1. Hematoxylin a. Alum b. Iron c. Other 2. Methylene blue 3. Celestine blue 4. Carmine B. Cytoplasmic 1. Eosin 2. Phloxine C. Combination 1. Hematoxylin and eosin 2. Giemsa 3. Methyl green-pyronin IV. Carbohydrates A. Carbohydrates classifications in tissue structures 1. Polysaccharides 2. Neutral mucins 3. Acid mucins 4. Sulfated and non-sulfated mucins 5. Mucoproteins 6. Glycoproteins B. Carbohydrate stains 1. Periodic acid Schiff (PAS) 2. Mucicarmine 3. Alcian blue stains 4. Colloidal iron stains 5. Congo red 6. Thioflavin T 7. Crystal violet/methyl violet V. Connective Tissue A. Collagen 1. Trichrome staining 2. Other B. Reticulum 1. Wilder 2. Gridley 3. Snook 4. Laidlaw C. Elastic 1. Verhoeff van Gieson 2. Aldehyde fuchsin 3. Orcein 4. Resorcin fuchsin D. Basement membranes 1. Jones 2. Gomori's PAS – Methenamine silver E. Muscle 1. Phosphotungstic Acid Hematoxylin (PTAH) 2. Trichromes 3. Lendrum VI. Lipids A. Tissue structures 1. Storage cells 2. Trauma displacement 3. Cellular degeneration B. Stains 1. Sudan black 2. Oil red O 3. Osmium tetroxide VII. Pigments A. Artifact 1. Formalin 2. Mercury 3. Chrome B. Exogenous 1. Carbon 2. Asbestos 3. Tattoo C. Endogenous hematogenous 1. Hemosiderin a. Prussian blue stain b. Turnbull's stain 2. Bile a. Hall's b. Other D. Endogenous nonhematogenous 1. Melanin a. Fontana-Masson b. Schmorl 2. Argyrophil granules a. Grimelius argyrophil stain b. Sevier-Munger c. Churukian-Schenk 3. Lipidic pigments a. Lipofucscin b. Ceroid VIII. Minerals A. Endogenous deposits 1. Urates – Gomori's methenamine silver 2. Other B. Calcium 1. Von Kossa 2. Alizarin red S C. Copper 1. Rhodanine 2. Rubeanic acid IX. Cytoplasmic Granules A. Chromaffin granules 1. Schmorl 2. Other B. Argentaffin granules 1. Fontana Masson 2. Other

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