MCCCD Official Course Competencies:

BIO209 2008 Fall - 2008 Fall

Biomolecular Separation

1.

Describe growth characteristics of bacterial, yeast and plant cells. (I)

2.

Describe conditions required for normal cell growth. (I)

3.

Apply biological concepts to grow bacterial, yeast and plant cells in culture. (I, II)

4.

Operate and troubleshoot apparatus used for cell culture. (II)

5.

Prepare and maintain viable cell cultures from various sources. (II, III)

6.

Use appropriate techniques to introduce nucleic acids into cells and monitor their expression. (IV)

7.

Separate synthesized proteins and other biomolecules using appropriate isolation techniques and check for purity and concentration. (IV)

MCCCD Official Course Outline:

BIO209 2008 Fall - 2008 Fall

Biomolecular Separation

I. Cell growth and culture

A. Introduction to cell metabolism

B. Growth characteristics of bacterial, yeast and plant cells

C. Conditions required for normal cell growth

II. Laboratory and industrial applications

A. Design, operation, monitoring and maintenance of equipment

B. Physical and physiological requirements for cell growth

1. Nutrients, media and lighting levels

2. pH

3. Dissolved oxygen and carbon dioxide concentrations

4. Osmolality and conductivity

C. Quality control and compliance

III. Cell and tissue culture techniques

A. Media preparation and maintenance

B. Sterile technique

IV. Use of cell culture to express heterologous DNA sequences

A. Introduction of nucleic acid sequences to form recombinant cells

1. Transformation

2. Electroporation

3. Other

B. Cell lysis

1. Enzymatic

2. Sonication

C. Characterization, purification and quantitation techniques from cellular homogenates or media

1. Centrifugation

2. Chromatography

3. Filtration

4. Gel electrophoresis

5. Spectrophotometry

6. Antibody detection

7. Enzyme assays

D. Scaling up production for industrial applications