| 1.
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Gather data, analyze data, and present data. (I)
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| 2.
|
Describe the correct use and application of basic equipment found in a
typical molecular or cellular biology laboratory. (I, II, XV)
|
| 3.
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Demonstrate proficiency in bacteriological technique. (I, IX)
|
| 4.
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Describe Beer's Law and Spectrophotometry. (II)
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| 5.
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Demonstrate proficiency with a light microscope. (II)
|
| 6.
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Describe the Gram stain (II)
|
| 7.
|
Demonstrate proficiency in protein and DNA purification (III)
|
| 8.
|
Purify both chromosomal and plasmid DNA. (III, VI, VIII, X)
|
| 9.
|
Define the central dogma of molecular biology. (IV)
|
| 10.
|
Demonstrate proficiency in transfomation of bacteria and yeast. (IV,
VII, IX)
|
| 11.
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Describe recombinant DNA techniques. (IV, VIII, IX)
|
| 12.
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Clone a segment of DNA (e.g., a gene) into a plasmid or expression
vector. (IV, IX)
|
| 13.
|
Measure and describe growth kinetics of a eukaryotic organism (yeast).
(V)
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| 14.
|
Describe allelic frequencies and the Hardy-Weinberg Equilibrium Theory
(VI)
|
| 15.
|
Develop a protocol, using genetic transformation techniques, that will
"correct" genetic deficiencies. (VII)
|
| 16.
|
Confirm identity of a DNA sample through restriction enzyme digestion
and mapping. (VIII, XI)
|
| 17.
|
Describe and perform nucleic acid hybridizations (southern blotting).
(XII)
|
| 18.
|
Describe PCR and design PCR primer. (XIII, XIV)
|
| 19.
|
Describe and perform maintenance of cultured mammalian cells. (XV)
|
| 20.
|
Describe the correct use of equipment found in a cell culture
laboratory (XV)
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|